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olig2 pe  (Novus Biologicals)


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    Novus Biologicals olig2 pe
    Olig2 Pe, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/olig2+pe/Olig2+Antibody+(OLIG2%2F2400)+%5BPE%5D/pm41173000-1045-40-41
    Average 93 stars, based on 1 article reviews
    olig2 pe - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Staining:

    Article Title: The brain neurovascular epigenome and its association with dementia.
    Article Snippet: .. Nuclei were stained in 600 μL FANS buffer overnight at 4◦C using combinations of the following antibodies: NEUN-488 (Merck Millipore, MAB377X) at 1:5000, OLIG2-488 at (Abcam, ab225099) at 1:5000, ERG-488 (Abcam, ab196374) at 1:100, PU1-PE (Cell Signaling, 81886) at 1:100, OLIG2-PE (Novus Biologicals, NBP3-08960PE) at 1:200, NOTCH3-PE (BioLegend, 345405) at 1:100, ERG-647 (Abcam, ab196149) at 1:100, PU1-647 (BioLegend, 658008 or Cell Signaling, 2240) at 1:100, OLIG2-647 (abcam, ab225100) at 1:5000 and RFX4 (abcam, ab188020) at 1:200. .. The next day, nuclei were washed with 3 mL FANS Buffer and centrifuged at 600xg at 4◦C for 10 min. For RFX4 staining, the nuclei pellet was resuspended with 600 μL FANS buffer and a secondary antirabbit PE antibody was added at 1:1000 (Cell Signaling, 79408), followed by a 2 h incubation on ice.



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    Image Search Results


    a NSCs were induced to differentiate into neurons/astrocytes/oligodendrocytes, and the cells were stained with NeuN (red)/GFAP (green)/Olig2 (yellow), collected and subjected to image flow cytometry. b Brightfield and darkfield (labelled) single-cell images were used as training data for the screening system; a schematic of the convolutional neural network (CNN) is presented. c Various inducers in different forms that act on different pathways were used to guide NSCs to differentiate into neurons, and brightfield (unlabelled) single-cell image patches were obtained by flow cytometry. These independent test sets were evaluated with the deep network model to show its generalizability.

    Journal: Nature Communications

    Article Title: Deep learning-based predictive identification of neural stem cell differentiation

    doi: 10.1038/s41467-021-22758-0

    Figure Lengend Snippet: a NSCs were induced to differentiate into neurons/astrocytes/oligodendrocytes, and the cells were stained with NeuN (red)/GFAP (green)/Olig2 (yellow), collected and subjected to image flow cytometry. b Brightfield and darkfield (labelled) single-cell images were used as training data for the screening system; a schematic of the convolutional neural network (CNN) is presented. c Various inducers in different forms that act on different pathways were used to guide NSCs to differentiate into neurons, and brightfield (unlabelled) single-cell image patches were obtained by flow cytometry. These independent test sets were evaluated with the deep network model to show its generalizability.

    Article Snippet: The cells were fixed in cold 80% methanol for 5 min and then disrupted using 0.1% Triton X-100 on ice for 20 min. Later, the cells were incubated with anti-GFAP (Bioss, catalogue no. bs-0199R-AF488, 1:200), anti-NeuN (Bioss, catalogue no. bs-10394R-APC, 1:200) and anti-Olig2 (Bioss, catalogue no. bs-11194R-PE, 1:200) antibodies for 2 h on ice.

    Techniques: Staining, Flow Cytometry

    Figure 4. Flow cytometry of 3D bioprinted NPCs after 15 days of culture in vitro (a) βT-III; (b) Olig2; (c) HB9. n = 3 for all groups. One-way ANOVA and Tukey post-hoc analysis was performed for statistical analysis using a confidence level of 95% (p < 0.05) and 99.9% (p < 0.001). * represents p < 0.05 and ** 99.9% p < 0.001. The same data visualized as a scatterplot can be seen in Supplementary Figure S2.

    Journal: Applied Sciences

    Article Title: 3D Bioprinting Human Induced Pluripotent Stem Cell-Derived Neural Tissues Using a Novel Lab-on-a-Printer Technology

    doi: 10.3390/app8122414

    Figure Lengend Snippet: Figure 4. Flow cytometry of 3D bioprinted NPCs after 15 days of culture in vitro (a) βT-III; (b) Olig2; (c) HB9. n = 3 for all groups. One-way ANOVA and Tukey post-hoc analysis was performed for statistical analysis using a confidence level of 95% (p < 0.05) and 99.9% (p < 0.001). * represents p < 0.05 and ** 99.9% p < 0.001. The same data visualized as a scatterplot can be seen in Supplementary Figure S2.

    Article Snippet: Flow Cytometry Cell marker expression of the early neuronal markers, βeta tubulin-III (βT-III) ((IC1195C R&D), oligodendrocyte transcription factor 2 (Olig2) (IC2230P R&D systems, Minneapolis, MN, USA), and the protein homeobox 9 (HB9) (bs-11320R, Bioss antibodies), was quantified on day 15.

    Techniques: Flow Cytometry, In Vitro